Peptide Conjugation: The key points that matters
Peptide synthesis is the order of the day since natural protein is scarce to be used in discovery and experiment purposes. We have a dire need to find a solution to fight the ailment of cancer as well as much other life-threatening diseases. All in all, peptides play an essential role in taking modern biotechnology research to a new horizon, providing researchers with better opportunities to carry forward the process of discovering new drugs to some specific diseases.
Bio-Synthesis is one of peptide conjugation service provider which offers a variety of immunogenic carrier proteins, enzymes, toxins, drugs or Oligonucleotides for of an immunogen or for affinity purification. A number of immunogenic carrier proteins like Keyhole Limpet Hemocyanin (KLH), Diphtheria Toxoid have been used in conjugation with several peptide chemistries. The company also offers coupling of peptides to different kinds of gel to develop an immunosorbent for positive or negative selection of macromolecular binding entries such as antibodies.
It is evident that target delivery involves the use of a biological vector which is s covalently bond and works as a therapeutic agent in drugs, biologic, toxins etc. Bio-Synthesis’s wide range of varieties also offers peptide-drug conjugation services. Peptide-drug conjugation contains peptide delivery vector, a therapeutic warhead and a linker that covalently links the vector and the warhead. Short peptides which are less than 40 amino acids are often used as peptide delivery vectors. For example, cell-penetrating peptides (CPPs) like Tat CPP peptides obtained from the HIV transcriptional activator protein. In our state-of-art laboratory, cell penetrating peptides are often used to conjugate to an antisense Oligonucleotide.
Service Specifications
To understand Peptide conjugation one needs to know the how peptide conjugation is performed using a variety of methods, depending upon the amino acid composition and project requirements. The N-terminal amine can be labeled, if no internal Lys residues are present, in the peptide. The C-terminal acid can be labeled if no Glu and Asp residues are present in the peptide. Artificial amino acids contain a functional group which is orthogonal (non-interfering) to peptide functional groups that can be incorporated during the process of peptide synthesis. Cys can also be added at the N-termini to incorporate thiol functionality if Cys residues are present, not present in peptide.
Chemistry: Peptide conjugation during N-terminal amine, C-terminal acid, Cys thiol, or other functional groups available.
Purification: The purification depends upon the type of molecules used for conjugation, most often low molecular weight contaminants will be abolished by SEC chromatography or HPLC.
Quality Control: Gel electrophoresis, MALDI MS (except for KLH) or other appropriate analysis.
Highlights:
Intelligent – Bio-Synthesis TAD Technology makes sure that carrier protein and peptide conjugation choices are selected intelligently and intentionally rather on mere presumption.
Cross-linking options – We have highly qualified professionals who are proficient with multiple conjugation chemistries and select combinations to pinpoint specific linkage sites on peptide antigens.
Carrier proteins –KLH and other accepted immunogenic carrier proteins are used, or specific choices in this regard made by our clients are taken into consideration.
Assay-specific – As we ensure quality, we help clients to select conjugation chemistries that best imitate epitope presentation in the intended assay method.
Epitope analysis – Bio-Synthesis advice the ways to maximize epitope exposure within structural motifs and orientation to the termini.
Design integration - together, our peptide design service (Antigen Profiler System) and carrier protein selection and conjugation method (TAD) maximize peptide solubility and linkage.
Bio-Synthesis is one of peptide conjugation service provider which offers a variety of immunogenic carrier proteins, enzymes, toxins, drugs or Oligonucleotides for of an immunogen or for affinity purification. A number of immunogenic carrier proteins like Keyhole Limpet Hemocyanin (KLH), Diphtheria Toxoid have been used in conjugation with several peptide chemistries. The company also offers coupling of peptides to different kinds of gel to develop an immunosorbent for positive or negative selection of macromolecular binding entries such as antibodies.
It is evident that target delivery involves the use of a biological vector which is s covalently bond and works as a therapeutic agent in drugs, biologic, toxins etc. Bio-Synthesis’s wide range of varieties also offers peptide-drug conjugation services. Peptide-drug conjugation contains peptide delivery vector, a therapeutic warhead and a linker that covalently links the vector and the warhead. Short peptides which are less than 40 amino acids are often used as peptide delivery vectors. For example, cell-penetrating peptides (CPPs) like Tat CPP peptides obtained from the HIV transcriptional activator protein. In our state-of-art laboratory, cell penetrating peptides are often used to conjugate to an antisense Oligonucleotide.
Service Specifications
To understand Peptide conjugation one needs to know the how peptide conjugation is performed using a variety of methods, depending upon the amino acid composition and project requirements. The N-terminal amine can be labeled, if no internal Lys residues are present, in the peptide. The C-terminal acid can be labeled if no Glu and Asp residues are present in the peptide. Artificial amino acids contain a functional group which is orthogonal (non-interfering) to peptide functional groups that can be incorporated during the process of peptide synthesis. Cys can also be added at the N-termini to incorporate thiol functionality if Cys residues are present, not present in peptide.
Chemistry: Peptide conjugation during N-terminal amine, C-terminal acid, Cys thiol, or other functional groups available.
Purification: The purification depends upon the type of molecules used for conjugation, most often low molecular weight contaminants will be abolished by SEC chromatography or HPLC.
Quality Control: Gel electrophoresis, MALDI MS (except for KLH) or other appropriate analysis.
Highlights:
Intelligent – Bio-Synthesis TAD Technology makes sure that carrier protein and peptide conjugation choices are selected intelligently and intentionally rather on mere presumption.
Cross-linking options – We have highly qualified professionals who are proficient with multiple conjugation chemistries and select combinations to pinpoint specific linkage sites on peptide antigens.
Carrier proteins –KLH and other accepted immunogenic carrier proteins are used, or specific choices in this regard made by our clients are taken into consideration.
Assay-specific – As we ensure quality, we help clients to select conjugation chemistries that best imitate epitope presentation in the intended assay method.
Epitope analysis – Bio-Synthesis advice the ways to maximize epitope exposure within structural motifs and orientation to the termini.
Design integration - together, our peptide design service (Antigen Profiler System) and carrier protein selection and conjugation method (TAD) maximize peptide solubility and linkage.
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