Merrifield solid phase peptide synthesis
Peptide Synthesis From the last 40 years or so Merrifield solid peptide synthesis has been a standard research procedure used in the further study of chemistry and biological usage of deamidation of asparaginyl and glutaminyl residues in peptides. In its primary investigation, initially, it permitted the qualitative demonstration in three different structure-determining deamidations which varied over a wide range in different biological conditions and the discovery of two biological systems took place in which deamidations works as molecular clocks. In the recent times, a thorough quantitative understanding of the structural dependence of deamidation became possible which results in deamidation rate of asparaginyl residues in protein can be predicted from three-dimensional structure of protein with a high degree of accuracy. In his investigation, Professor R.B. Merrifield discovered that amide residues serve as molecular clock in many biological systems. It is this technique on w...